Cellular Aggregation in the Platelet-Tumor Cell Microenvironment
(A) Aggregation trace depicts typical agonist-induced platelet aggregation (10 μM adenosine diphosphate (ADP), and 1 mM arachidonic acid [AA]) and inhibition by antiplatelet agents (A and B) ticagrelor (Ticag) (10 μM) and aspirin (ASA) (50 μM), respectively. Platelets from normal healthy donors were pre-incubated with Ticag (10 μM), ASA (50 μM), or dual inhibitors, then stirred in the presence of (C) MCF-7 breast cancer cells or (D) HT-29 colorectal cancer cells. Values are mean percent aggregation ± SEM (n = 8). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. DAPT = dual antiplatelet therapy (ticagrelor and aspirin); Plts = platelets; TC = tumor cells.